ABSTRACT
Introduction: Nucleic acid testing (NAT) is the latest approved method for detection of transfusion transmitted infections. With higher prevalence of infections in window period and occult infections, blood safety can be compromised when testing is done using only serological techniques. With this background this study had been taken up to compare the results of serological test with NAT. Material and methods: Voluntary blood donations were screened for hepatitis B, hepatitis C and human immunodeficiency virus using ELISA. All samples were sent for ID-NAT and results of both methods were compared, analyzed individually. Results: A total of 11,393 blood units were collected during the study period. ELISA test results. showed 176 (1.54%) positivity for these transfusion transmitted infections (TTIs) in total. Among them, 133 (75.56%) was HBV, 22 (12.5%) was HCV and 21 (11.93%) was HIV as shown by ELISA. ID- NAT test results showed 174 (1.52%) positive in total. As analyzed by individual test results, 37 (0.32%) of total blood samples showed positivity in NAT which showed negative results in ELISA. Also 38 (0.33%) of total blood samples (HBV = 27, HCV = 08, HIV = 03) which showed positivity in ELISA were negative in NAT. Conclusion: ID-NAT is definitely a useful screening method for HBV, HCV, HIV. It clarifies infections in window period, occult infections and false seroreactive cases.
ABSTRACT
Fibromatosis is a group of disorders characterized by infiltrating fibroblastic proliferation. Among them Juvenile hyaline fibromatosis (J.H.F) is a rare, progressive, crippling autosomal recessive disorder diagnosed based on the characteristic clinicopathological findings of generalized cutaneous nodular lesions, gingival hypertrophy, flexion contractures of large joints with osteolytic lesions, and proliferating fibroblasts set within a hyalinized stroma. This disorder commonly manifests in childhood with family history of consanguinity and in siblings. A case of seven year old boy born to consanguinous parents is reported who presented with multiple subcutaneous nodules and gingival hypertrophy. Histopathological examination revealed proliferating fibroblasts embedded in an abundant homogeneous eosinophilic hyalinized matrix. The matrix showed PAS stain positivity, supporting the pathogenesis of this disorder as an inborn error of glycosaminoglycans metabolism. The differential diagnosis is discussed and the literature reviewed.